Molecular cloning and expression of active Ole e 3, a major allergen from olive‐tree pollen and member of a novel family of Ca²⁺‐binding proteins (polcalcins) involved in allergy

Abstract excerpt

A cDNA encoding Ole e 3, a major allergen from olive-tree pollen, has been cloned and sequenced. A strategy based on two-step PCR amplification towards the 5' end and 3' end, with an internal specific primer, has been used. The isolated cDNA contains an open reading frame coding for a polypeptide of 84 amino acids, which is in agreement with the composition and molecular mass of the natural allergen, exhibiting...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Allergic Rhinitis and Sensitization
• Share to Contact Dermatitis and Allergies
• Share to Food Allergy and Anaphylaxis Research