Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

doi:10.1023/a:1008874129407

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Article

Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

Transgenic research - 1 Jul 1998

Chowrira G M, Cavileer T D, Gupta S K, Lurquin P F, Berger P H

Abstract excerpt

Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants...

Topics

Base Sequence
Capsid
DNA Primers
Gene Expression
Genes, Viral
Mosaic Viruses
Pisum sativum
Phenotype
Plants, Genetically Modified
Polymerase Chain Reaction
Transformation, Genetic
Virulence

Source

Open in PubMed

Identifiers

PMID: 9859215
DOI: 10.1023/a:1008874129407

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