Article
Development of a multiplex PCR gene fingerprinting method using gyrA and pflA polymorphisms to identify genotypic relatedness within Campylobacter jejuni species.
Journal of applied microbiology - 1 Nov 1998
Ragimbeau C, Salvat G, Colin P, Ermel G
Abstract excerpt
The two genes gyrA and pflA, whose sequence variability had been previously described, were evaluated separately for their potential value in discriminating strains of Campylobacter jejuni. A single method was then developed by which the two loci were simultaneously amplified using a multiplex PC...
Topics
- Animals
- Bacterial Proteins
- Campylobacter jejuni
- Cattle
- Chickens
- Cluster Analysis
- DNA Fingerprinting
- DNA Gyrase
- DNA Topoisomerases, Type II
- Electrophoresis, Gel, Pulsed-Field
- Flagellin
- Genes, Bacterial
- Genotype
- Humans
- Membrane Proteins
- Polymerase Chain Reaction
- Restriction Mapping