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Article

Development of a multiplex PCR gene fingerprinting method using gyrA and pflA polymorphisms to identify genotypic relatedness within Campylobacter jejuni species.

Journal of applied microbiology - 1 Nov 1998

Ragimbeau C, Salvat G, Colin P, Ermel G

Abstract excerpt

The two genes gyrA and pflA, whose sequence variability had been previously described, were evaluated separately for their potential value in discriminating strains of Campylobacter jejuni. A single method was then developed by which the two loci were simultaneously amplified using a multiplex PCR procedure, and banding patterns were generated using a pre-selected set of restriction endonuclease enzymes. The...

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PMID: 9830118
DOI: 10.1046/j.1365-2672.1998.00597.x

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