Article
Typing of HLA-B*15 alleles using sequence-specific primers.
Tissue antigens - 1 Sept 1998
Yu N, Ohashi M, Alosco S, Salazar M, Cao K, Fernandez Vina M, Yunis E J
Abstract excerpt
We have developed a DNA based typing method to detect 38 known B*15 alleles using sequence-specific primers (PCR-SSP). This method involves 38 primers and 39 PCR-SSP reactions with results that can be obtained in 3 hours. The method is easy, fast and suitable for clinical typing for bone marrow and organ transplantation. We have typed 106 HLA-B15 samples using this method. For homozygous HLA-B15 samples, some...
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