Determination of Serotypes of Astroviruses by Reverse Transcription‐Polymerase Chain Reaction and Homologies of the Types by the Sequencing of Japanese Isolates

Abstract excerpt

Human standard astroviruses, serotypes 1 to 7, and 35 Japanese isolates were typed by reverse transcription and polymerase chain reaction (RT-PCR) with serotype-specific primers for the first time. The results were identical with those obtained by enzyme immunoassay with serotype-specific polyclonal antibodies, a method which has already been reported. RT-PCR with serotype-specific primers is useful for...

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