Article
Fluorescent amplified-fragment length polymorphism analysis of an outbreak of group A streptococcal invasive disease.
Journal of clinical microbiology - 1 Nov 1998
Desai M, Tanna A, Wall R, Efstratiou A, George R, Stanley J
Abstract excerpt
Fluorescent amplified-fragment length polymorphism (FAFLP) analysis was carried out for an outbreak of group A streptococcal (GAS) invasive disease. Streptococcal genomic DNAs were digested with endonucleases EcoRI and MseI, site-specific adaptors were ligated, and PCR amplification was carried o...
Topics
- Aged
- Base Sequence
- DNA Primers
- DNA, Bacterial
- Disease Outbreaks
- Electrophoresis, Gel, Pulsed-Field
- Fluorescent Dyes
- Genotype
- Humans
- London
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
