Simultaneous analysis of various mutations on the 21-hydroxylase gene by multi-allele specific amplification and capillary gel electrophoresis.

doi:10.1016/s0021-9673(98)00457-9

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Article

Simultaneous analysis of various mutations on the 21-hydroxylase gene by multi-allele specific amplification and capillary gel electrophoresis.

Journal of chromatography. A - 21 Aug 1998

Barta C, Sasvári-Székely M, Guttman A

Abstract excerpt

A detailed study is presented on the detection of various known point mutations using polymerase chain reaction (PCR) based multi-allele specific amplification (MASA) in conjunction with capillary gel electrophoresis (CGE) separation. The resulting PCR products, corresponding to the individual mu...

Topics

Alleles
Bacteriophage phi X 174
Base Sequence
DNA Primers
Electrophoresis, Capillary
Fluorescent Dyes
Mutation
Polymerase Chain Reaction
Restriction Mapping
Steroid 21-Hydroxylase

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