Article
Rapid deoxyribonucleic acid analysis by allele-specific polymerase chain reaction for detection of mutations in the steroid 21-hydroxylase gene.
The Journal of clinical endocrinology and metabolism - 1 May 1995
Wilson R C, Wei J Q, Cheng K C, Mercado A B, New M I
Abstract excerpt
Rapid DNA analysis based on allele-specific polymerase chain reaction (PCR) using mutation site-specific primers was developed to detect mutations in the CYP21 gene known to cause steroid 21-hydroxylase deficiency. In contrast to the previous method, in which PCR of genomic DNA was followed by do...
Topics
- Alleles
- Base Sequence
- DNA
- Female
- Genes
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Steroid 21-Hydroxylase
- Time Factors