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Development and use of 16S rRNA gene targeted PCR primers for the identification of <i>Escherichia coli</i> cells in water

1 Sept 1998

Abstract excerpt

The primary sequences of the V3 and V6 regions of the 16S rRNA gene of pathogenic and non-pathogenic strains of Escherichia coli were determined and compared with those obtained for a number of reference strains which belong to the family Enterobacteriaceae. Three oligonucleotide primers 16E1, 16E2 and 16E3 were designed and used in the polymerase chain reaction to identify specifically all E. coli isolates. When...

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PMID: 9750286
DOI: 10.1046/j.1365-2672.1998.853535.x

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