Article
[PCR analysis of microsatellite DNA markers: possibility of erroneous determination of genotypes].
Genetika - 1 Jun 1998
Popova S N, Shadrina M I, Fatkhlislamova R I, Khusnutdinova E K, Slominskiĭ P A
Abstract excerpt
A comparative study of two techniques for the PCR genotyping of highly polymorphic tandem repeats was carried out by the example of a triplet repeat in the myotonin protein kinase gene. Sequencing denaturing gels were shown to yield more precise results in the analysis of amplification products.
Topics
- DNA, Satellite
- Electrophoresis, Polyacrylamide Gel
- Genetic Markers
- Genotype
- Myotonin-Protein Kinase
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Protein Kinases
- Protein Serine-Threonine Kinases
- Repetitive Sequences, Nucleic Acid
