Article
The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia.
Parasitology - 1 Jul 1998
Victoir K, Bañuls A L, Arevalo J, Llanos-Cuentas A, Hamers R, Noël S, De Doncker S, Le Ray D, Tibayrenc M, Dujardin J C
Abstract excerpt
In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63-RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR-RFLP). Both methods were a...
Topics
- Animals
- Blotting, Southern
- Electrophoresis, Agar Gel
- Electrophoresis, Cellulose Acetate
- Genetic Variation
- Glycoproteins
- Isoenzymes
- Leishmania
- Leishmaniasis
- Nucleic Acid Hybridization
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Restriction Mapping
- South America