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Nested PCR Assay for Detection of Granulocytic Ehrlichiae

1 Apr 1998

Abstract excerpt

A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis. Five of the seven suspected cases were positive by the PCR assay using DNA extracted from whole blood as...

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PMID: 9542943
DOI: 10.1128/jcm.36.4.1090-1095.1998

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