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PCR Detection of Acute Ehrlichia Canis Infection in Dogs

1 Oct 1996

Abstract excerpt

A polymerase chain reaction (PCR)-based detection assay that specifically detected Ehrlichia canis in dogs with acute infections was developed. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification and chemiluminescent hybridization (CH) with a complementary internal 287-base pair (bp) oligonucleotide probe. The CH improved the PCR assay sensitivity 1,000-fold as compared with...

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PMID: 8953528
DOI: 10.1177/104063879600800406

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