Article
Genotypic analysis of the 5'-untranslated region of a pestivirus strain isolated from human leucocytes.
Microbiology and immunology - 1 Jan 1997
Giangaspero M, Harasawa R, Verhulst A
Abstract excerpt
The 5'-untranslated genomic region of the pestivirus strain Europa, originated in human leucocytes and previously identified as bovine diarrhea virus (BVDV), was amplified by reverse transcription-PCR and sequenced. Analyses based on primary nucleotide sequence homology and on secondary palindromic sequence structures characteristic to genotypes revealed that this human isolate should be assigned to a novel...
Topics
- Animals
- Cattle
- DNA Primers
- Diarrhea Viruses, Bovine Viral
- Genotype
- Humans
- Leukocytes
- Molecular Sequence Data
- Nucleic Acid Conformation
- Pestivirus
- Phylogeny
- Polymerase Chain Reaction
- RNA, Viral
- Sequence Analysis, RNA
- Sequence Homology, Nucleic Acid
