Polymerase chain reaction detection and speciation of <b> <i>Campylobacter upsaliensis</i> </b> and <b> <i>C. helveticus</i> </b> in human faeces and comparison with culture techniques

Abstract excerpt

A polymerase chain reaction (PCR) assay based on the 16S rRNA gene and an improved DNA extraction procedure were developed for the direct detection and differentiation of Campylobacter upsaliensis and C. helveticus in seeded human faeces. The PCR assay was compared with culture detection by a membrane filter (MF) technique and on selective agar (SA) containing 8 mg l-1 cefoperazone. Both MF culture and the PCR...

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