Article
Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Apr 1997
Kishida T, Wang W, Fukuda M, Tamaki Y
Abstract excerpt
Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex...
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