Article
Characterization of microsatellite markers in eastern white pine.
Genome - 1 Dec 1996
Echt C S, May-Marquardt P, Hseih M, Zahorchak R
Abstract excerpt
An enrichment cloning method was evaluated for the isolation of microsatellite loci from eastern white pine and the resulting markers were examined for polymorphisms. A 200-fold enrichment was achieved for highly abundant (AC)n repeats, but for much less abundant (ACAG)n repeats an enrichment of only 20-fold was obtained. Using a single set of PCR conditions, 19 microsatellite loci were identified from 77 primer...
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