Article
Rapid and simple diagnosis of the two common alpha 1-proteinase inhibitor deficiency alleles Pi*Z and Pi*S by DNA analysis.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Sept 1996
Braun A, Meyer P, Cleve H, Roscher A A
Abstract excerpt
We describe a simple DNA-based method to assign the two common alpha 1-proteinase inhibitor (alpha 1-antitrypsin) deficiency alleles in the Pi-system (Pi*Z and Pi*S). Two sets of mutated primers are used in the polymerase chain reaction (PCR), followed by a restriction enzyme digest of the produc...
Topics
- Alleles
- Base Sequence
- DNA Mutational Analysis
- Electrophoresis, Agar Gel
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- alpha 1-Antitrypsin
- alpha 1-Antitrypsin Deficiency