Article
An investigation into the use of SDS-PAGE of cell surface extracts and proteolytic activity to differentiate Prevotella nigrescens and Prevotella intermedia.
FEMS microbiology letters - 15 Feb 1996
Cookson A L, Wray A, Handley P S, Jacob A E
Abstract excerpt
By comparison of the cell surface proteins derived from the outer membrane and fibrils from 14 Prevotella intermedia and 19 Prevotella nigrescens strains using SDS and analysed by SDS-PAGE, it was possible to distinguish the two species. A polypeptide of approx. 21 kDa distinguished P. intermedia...
Topics
- Bacterial Outer Membrane Proteins
- Cell Extracts
- Coloring Agents
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Gingival Diseases
- Humans
- Peptides
- Periodontal Diseases
- Phenotype
- Prevotella
- Prevotella intermedia
- Sodium Dodecyl Sulfate