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Microtubule and Chromatin Dynamics during Fertilization and Early Development in Rhesus Monkeys, and Regulation by Intracellular Calcium Ions1

1 Aug 1996

Abstract excerpt

To explore primate fertilization, oocytes and zygotes from fertile rhesus monkeys were imaged throughout fertilization, polyspermy, and artificial activation using confocal microscopy for microtubules and DNA, as well as ratiometric computer-enhanced video microscopy for intracellular calcium. Unfertilized oocytes displayed microtubules only in the radially oriented meiotic spindles. At insemination, a large...

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PMID: 8828828
DOI: 10.1095/biolreprod55.2.260

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