Molecular analysis of isolates of Salmonella typhi obtained from patients with fatal and nonfatal typhoid fever.

doi:10.1128/jcm.34.4.1029-1033.1996

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Article

Molecular analysis of isolates of Salmonella typhi obtained from patients with fatal and nonfatal typhoid fever.

Journal of clinical microbiology - 1 Apr 1996

Thong K L, Passey M, Clegg A, Combs B G, Yassin R M, Pang T

Abstract excerpt

Molecular characterization of a total of 52 human isolates of Salmonella typhi from Papua New Guinea was performed by using pulsed-field gel electrophoresis (PFGE) after digestion of chromosomal DNA with three restriction endonucleases, XbaI (5'-TCTAGA-3'), AvrII (5'-CCTAGG-3'), and SpeI (5'-ACTAGT-3'). Of the 52 isolates tested, 11 were obtained from patients with fatal typhoid fever and 41 were obtained from...

Topics

Base Sequence
DNA Restriction Enzymes
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Genetic Variation
Genotype
Humans
Molecular Biology
Papua New Guinea
Salmonella typhi
Typhoid Fever
Virulence

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Identifiers

PMID: 8815078
DOI: 10.1128/jcm.34.4.1029-1033.1996
PMCID: PMC228948

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