Article
Allele assignment for HLA-A, -B, and -C genes to the Tenth International Histocompatibility Workshop cell lines.
Tissue antigens - 1 Jun 1996
Prasad V K, Yang S Y
Abstract excerpt
Development of DNA typing for Class I HLA alleles has lagged behind that of class II for a variety of technical reasons. Following the recognition of locus specific sequences in the first and the third intron, and acquiring the ability to amplify genomic DNA by intron-based PCR primer, we have devised DNA typing of class I alleles by SSOP and direct sequencing. In this study using these techniques we provide the...
Topics
- Alleles
- Cell Line, Transformed
- Education
- HLA-A Antigens
- HLA-B Antigens
- HLA-C Antigens
- Histocompatibility
- Humans