Article
Quantitative multistandard RT-PCR assay using interspecies polymorphism.
Molecular and cellular probes - 1 Jun 1996
Khiri H, Reynier P, Peyrol N, Lerique B, Torresani J, Planells R
Abstract excerpt
The use of RT-PCR to quantify mRNA is often compromised by the variability of reverse-transcription and amplification reactions as well as by the difficulty of assessing the amount and/or the integrity of input RNA. Use of a competitor RNA or the coamplification of an endogenous standard are widespread methods of monitoring these steps. Taking advantage of both sequence conservation between homologous genes in...
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