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Article

Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900.

Journal of clinical microbiology - 1 Jul 1996

Bauerfeind R, Benazzi S, Weiss R, Schliesser T, Willems H, Baljer G

Abstract excerpt

Mycobactin J-dependent mycobacterial isolates from sheep, goat, and cattle herds with Johne's disease in Morocco, South Africa, the United States, and Germany were tested for the repetitive insertion sequence IS900 of Mycobacterium paratuberculosis by PCR. The IS900 PCR target sequence was detected in 90 of 93 fecal culture isolates tested (96.8%). Restriction fragment length polymorphisms (RFLPs) and in vitro...

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Identifiers

PMID: 8784556
DOI: 10.1128/JCM.34.7.1617-1621.1996
PMCID: PMC229081

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