Article
Diversity of host species and strains of Pneumocystis carinii is based on rRNA sequences.
Clinical and diagnostic laboratory immunology - 1 Jan 1996
Shah J S, Pieciak W, Liu J, Buharin A, Lane D J
Abstract excerpt
We have amplified by PCR Pneumocystis carinii cytoplasmic small-subunit rRNA (variously referred to as 16S-like or 18S-like rRNA) genes from DNA extracted from bronchoalveolar lavage and induced sputum specimens from patients positive for P. carinii and from infected ferret lung tissue. The amplification products were cloned into pUC18, and individual clones were sequenced. Comparison of the determined sequences...
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