Comparison of cell culture, mouse inoculation, and PCR for detection of Toxoplasma gondii: effects of storage conditions on sensitivity

Abstract excerpt

The sensitivity of detection of a wild-type strain of Toxoplasma gondii by cell culture, mouse inoculation, and PCR was determined following sample storage under conditions to which clinical specimens may be subjected during transport to the testing laboratory. Sample storage at -20 degrees C significantly decreased the sensitivity of mouse inoculation. The sensitivity of cell culture decreased with sample...

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