Article
Site-directed mutagenesis of human type X collagen. Expression of alpha1(X) NC1, NC2, and helical mutations in vitro and in transfected cells.
The Journal of biological chemistry - 7 Jun 1996
Chan D, Weng Y M, Hocking A M, Golub S, McQuillan D J, Bateman J F
Abstract excerpt
Type X collagen is a short chain collagen expressed in the hypertrophic zone of calcifying cartilage during skeletal development and bone growth. The alpha1(X) homotrimer consists of three protein domains, a short triple helix (COL1) flanked by nonhelical amino-terminal (NC2) and carboxyl-termina...
Topics
- Animals
- Base Sequence
- Cell Line
- Collagen
- DNA Primers
- DNA, Complementary
- Gene Expression
- Humans
- In Vitro Techniques
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis, Site-Directed
- Mutation
- Osteochondrodysplasias
- Point Mutation
- Protein Biosynthesis
- Protein Structure, Secondary
- Rats