Article
INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors.
The Journal of neuroscience : the official journal of the Society for Neuroscience - 1 May 1996
Hardie R C
Abstract excerpt
Absolute Ca2+ levels in dissociated Drosophila photoreceptors were measured using the ratiometric indicator dye INDO-1 loaded via patch pipettes, which simultaneously recorded whole-cell currents. In wild-type photoreceptors, the ultraviolet (UV) excitation light used to measure fluorescence elicited a massive Ca2+ influx that saturated the dye (>10 microM Ca2+), but lagged the electrical response by 2.8 msec....
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