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PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples

1 Dec 1995

Abstract excerpt

A PCR and a reverse cross blot hybridization assay were developed for the detection and identification of mycobacteria in clinical samples. The PCR amplifies a part of the DNA coding for 16S rRNA with a set of primers that is specific for the genus Mycobacterium and that flanks species-specific sequences within the genes coding for 16S rRNA. The PCR product is analyzed in a reverse cross blot hybridization assay...

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PMID: 8586707
DOI: 10.1128/jcm.33.12.3225-3233.1995

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