Genus-specific polymerase chain reaction for the mycobacterial dnaJ gene and species-specific oligonucleotide probes

Abstract excerpt

Identification of tuberculous and nontuberculous mycobacteria by biochemical methods is a long-term process that takes up to 8 weeks for completion and requires expertise to interpret the results. In order to detect and differentiate the major pathogenic mycobacterial species, we developed genus-specific primers that amplify the dnaJ gene from the broad spectrum of mycobacterial species and determined the...

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