Article
Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes.
Journal of clinical microbiology - 1 Dec 1995
Rijpkema S G, Molkenboer M J, Schouls L M, Jongejan F, Schellekens J F
Abstract excerpt
We developed a rapid and reliable method for the identification Borrelia burgdorferi sensu lato species in ticks. We used the DNA sequence polymorphism of the spacer region between 5S and 23S rRNA genes, which has been shown to be able to discriminate between eight genomic groups of B. burgdorfer...
Topics
- Animals
- Bacterial Typing Techniques
- Base Sequence
- Borrelia
- Borrelia burgdorferi Group
- DNA Primers
- DNA, Bacterial
- DNA, Ribosomal
- Genes, Bacterial
- Genotype
- Humans
- Ixodes
- Lyme Disease
- Molecular Sequence Data