Article
A linkage map with microsatellites isolated from swine flow-sorted chromosome 11.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Sept 1995
Riquet J, Milan D, Woloszyn N, Schmitz A, Pitel F, Frelat G, Gellin J
Abstract excerpt
We have developed a simple and efficient method to construct partial libraries of swine Chromosome (Chr) 11, starting with only 300 flow-sorted copies. DNA is amplified by PARM-PCR with primer containing at the 5'-end the sequence AGCU-. After amplification, digestion of PCR products with uracil DNA glycosylase generates cohesive ends corresponding to the SstI site. The amplified fragments can then be ligated in...
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