Article
Microsatellite markers from a microdissected swine chromosome 6 genomic library.
Animal genetics - 1 Aug 1999
Zhao F, Ambady S, Ponce de León F A, Miller L M, Lunney J K, Grimm D R, Schook L B, Louis C F
Abstract excerpt
To develop additional microsatellite (MS) markers in the region of the porcine skeletal muscle ryanodine receptor gene (RYR1), a microdissected genomic library was generated from the proximal half of the q arm of swine chromosome 6. Purified DNA was restriction enzyme-digested, ligated to oligonucleotide adaptors and amplified by PCR using primers complementary to the adaptor sequences. The purity of the...
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