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Diagnosis of Rabies by Polymerase Chain Reaction with Nested Primers

1 Jan 1993

Abstract excerpt

A simple, sensitive, and specific polymerase chain reaction (PCR) protocol for detection of rabies virus is described. The process consists of sample preparation, reverse transcription, two-step DNA amplification, and detection of the amplified product. RNA was extracted from animal and human brain by phenol-chloroform using guanidinium thiocyanate. Viral RNA was then amplified in a two-step PCR that used two...

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PMID: 8418168
DOI: 10.1093/infdis/167.1.207

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