Article
A heminested polymerase chain reaction for the detection of Brazilian rabies isolates from vampire bats and herbivores.
Memorias do Instituto Oswaldo Cruz - 1 Jan 2002
Soares R M, Bernardi F, Sakamoto S M, Heinemann M B, Cortez A, Alves L M, Meyer A D, Ito F H, Richtzenhain L J
Abstract excerpt
A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directed to highly conserved regions. All 42 clinical samples positive by both fluorescent antibody and mouse inoculation...
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