Article
Characterization of the suprachiasmatic nucleus in organotypic slice explant cultures.
Microscopy research and technique - 1 May 1993
Wray S, Castel M, Gainer H
Abstract excerpt
Suprachiasmatic nuclei (SCN) from hypothalami of postnatal rats were maintained for 18-39 days in vitro as organotypic slice explants. Neuronal subtypes containing vasopressin (VP), vasoactive intestinal polypeptide (VIP), gastrin releasing hormone (GRP), and GABA were immunocytochemically identi...
Topics
- Animals
- DNA Probes
- Female
- Gastrin-Releasing Peptide
- Histocytochemistry
- Humans
- In Situ Hybridization
- Neurons
- Organ Culture Techniques
- Peptides
- Phenotype
- RNA, Messenger
- Rats
- Rats, Sprague-Dawley
- Suprachiasmatic Nucleus
- Vasoactive Intestinal Peptide
- Vasopressins
- gamma-Aminobutyric Acid