Article
A fluorescence-based quantitative adhesion assay to study interactions between Entamoeba histolytica and human enterocytes. Effect of proinflammatory cytokines.
Journal of immunological methods - 3 Dec 1993
Flores-Romo L, Bacon K B, Estrada-Garcia T, Shibayama M, Tsutsumi V, Martinez-Palomo A
Abstract excerpt
In order to study the initial events during infection of target cells by the enteric pathogen Entamoeba histolytica, we developed a quantitative adhesion assay based on the use of a human colonic cell line (CaCo-2) and biotinylated amoebae tagged with fluorescein. To prevent the strong and rapid...
Topics
- Animals
- Cell Adhesion
- Cell Separation
- Cells, Cultured
- Colon
- Cytokines
- Entamoeba histolytica
- Flow Cytometry
- Fluorescein-5-isothiocyanate
- Fluorescence
- Host-Parasite Interactions