A fluorescence-based quantitative adhesion assay to study interactions between Entamoeba histolytica and human enterocytes. Effect of proinflammatory cytokines.

Abstract excerpt

In order to study the initial events during infection of target cells by the enteric pathogen Entamoeba histolytica, we developed a quantitative adhesion assay based on the use of a human colonic cell line (CaCo-2) and biotinylated amoebae tagged with fluorescein. To prevent the strong and rapid lytic activity of Entamoeba histolytica on colonic cells, which would otherwise impede the study of the primary...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cell Adhesion
• Share to Cell Separation