Article
Functional characterization of the cloned human ACTH receptor: impaired responsiveness of a mutant receptor in familial glucocorticoid deficiency.
Biochemical and biophysical research communications - 30 Nov 1993
Weber A, Kapas S, Hinson J, Grant D B, Grossman A, Clark A J
Abstract excerpt
The putative ACTH receptor gene has been identified on the basis of its tissue specific expression, structure, and limited expression data. We have expressed this gene in COS-7 cells and measured cAMP production in response to ACTH. An EC50 of 5.5 x 10(-9) M for ACTH (1-24) was determined. The S7...
Topics
- Adrenocorticotropic Hormone
- Animals
- Causality
- Cells, Cultured
- Cloning, Molecular
- Cyclic AMP
- Dose-Response Relationship, Drug
- Glucocorticoids
- Humans
- Mutation
- Receptors, Corticotropin
- Recombinant Proteins