Article
The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1993
Long D, Martin M, Sundberg E, Swinburne J, Puangsomlee P, Coupland G
Abstract excerpt
A two-component transposon system based on the Ac element of maize was used as a mutagen in Arabidopsis thaliana. Transposition of a Ds element marked with a hygromycin-resistance gene was activated from four different locations in the Arabidopsis genome. The progeny of 201 plants carrying independent transposition events were screened for mutants with severe, visible phenotypes. Seven mutants were identified and...
Topics
- Arabidopsis
- Base Sequence
- DNA
- DNA Primers
- DNA Transposable Elements
- Glucuronidase
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Polymerase Chain Reaction