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Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and in saliva

1 Sept 1993

Abstract excerpt

The nested polymerase chain reaction (PCR) technique was applied to investigate hepatitis C virus (HCV) RNA in the peripheral blood mononuclear cells (PBMCs), saliva, and serum of patients with chronic type C hepatitis. The specificity of the amplified products was analyzed and confirmed by agarose gel electrophoresis, Southern blot hybridization, and restriction endonuclease pattern analysis. HCV RNA was...

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PMID: 8228938
DOI: 10.1002/jmv.1890410112

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