Article
Unique oligonucleotide primers in PCR for identification of Cryptococcus neoformans
1 Jan 1994
Abstract excerpt
On the basis of a comparison of rRNA sequences coding the internal transcribed spacer (ITS) and 5.8S regions of Filobasidiella neoformans, the teleomorph of Cryptococcus neoformans, and members of its most closely related taxa, unique oligonucleotides were designed for the specific amplification of DNA only from C. neoformans. Various combinations of these primers, designated CN4, CN5, and CN6, and the previously...
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