Article
Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.
PCR methods and applications - 1 Dec 1993
Pecharatana S, Pickett M A, Watt P J, Ward M E
Abstract excerpt
A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems...
Topics
- Base Sequence
- Chlamydia Infections
- Chlamydia trachomatis
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Eye Infections, Bacterial
- Fluorescent Dyes
- Gambia
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction