Article
Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.
PCR methods and applications - 1 Dec 1993
Pecharatana S, Pickett M A, Watt P J, Ward M E
Abstract excerpt
A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B...
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