Article
Cloning and restriction fragment length polymorphism analysis of a cDNA for swine PIT-1, a gene controlling growth hormone expression.
Animal genetics - 1 Feb 1993
Tuggle C K, Yu T P, Helm J, Rothschild M F
Abstract excerpt
A partial swine cDNA which encodes the functional domain of PIT-1 was isolated by the polymerase chain reaction (PCR). The swine PIT-1 cDNA clone is 95% identical at the protein level to the rat Pit-1 gene. Thus, Pit-1's known function in control of rat growth hormone and prolactin expression is...
Topics
- Amino Acid Sequence
- Animals
- Base Composition
- Base Sequence
- Blotting, Southern
- Cerebral Cortex
- Cloning, Molecular
- DNA
- DNA-Binding Proteins
- Electrophoresis, Agar Gel
- Genes, Regulator
- Genetic Variation
- Molecular Sequence Data
- Pituitary Gland
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sequence Homology, Amino Acid
- Swine