PCR-based assays of mendelian polymorphisms from anonymous single-copy nuclear DNA: techniques and applications for population genetics.

doi:10.1093/oxfordjournals.molbev.a040002

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Article

PCR-based assays of mendelian polymorphisms from anonymous single-copy nuclear DNA: techniques and applications for population genetics.

Molecular biology and evolution - 1 Mar 1993

Karl S A, Avise J C

Abstract excerpt

This paper outlines a PCR-based approach for population genetics that offers several advantages over conventional Southern blotting methods for revealing restriction-fragment-length polymorphisms (RFLPs) in nuclear DNA. Primers are constructed from clones isolated from a nuclear DNA library, and...

Topics

Animals
Base Sequence
Cell Nucleus
DNA
Enzymes
Gene Library
Genetic Markers
Genetics, Population
Genotype
Male
Molecular Sequence Data
Ostreidae
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Turtles

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