Article
Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions.
Applied and environmental microbiology - 1 May 1994
Van Dyk T K, Majarian W R, Konstantinov K B, Young R M, Dhurjati P S, LaRossa R A
Abstract excerpt
Heat shock gene expression is induced by a variety of environmental stresses, including the presence of many chemicals. To address the utility of this response for pollutant detection, two Escherichia coli heat shock promoters, dnaK and grpE, were fused to the lux genes of Vibrio fischeri. Metals...
Topics
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Environmental Monitoring
- Escherichia coli
- Escherichia coli Proteins
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Light
- Luminescence
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutation
- Vibrio
- Water Pollutants, Chemical