Isolation of a Bacillus subtilis spoIIGA allele that suppresses processing-negative mutations in the Pro-sigma E gene (sigE).

Abstract excerpt

sigma E, a sporulation-essential sigma factor of Bacillus subtilis, is formed by a developmentally regulated proteolysis which removes 27 to 29 amino acids from the amino terminus of an inactive precursor protein (Pro-sigma E). A mutation which facilitates the conversion of inefficiently processed Pro-sigma E variants into mature sigma E was identified and mapped to spoIIGA. The isolation of such a mutation...

Topics

• Alleles
• Amino Acid Sequence
• Bacillus subtilis
• Bacterial Proteins
• Membrane Proteins
• Molecular Sequence Data
• Mutation
• Peptide Hydrolases
• Protein Processing, Post-Translational
• Recombinant Fusion Proteins
• Sequence Homology, Amino Acid