Article
Phenotypes of Bacillus subtilis mutants altered in the precursor-specific region of sigma E.
Journal of bacteriology - 1 Aug 1990
Jonas R M, Peters H K, Haldenwang W G
Abstract excerpt
sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is synthesized from an inactive precursor protein (P31). The structural gene (sigE) for P31 was reengineered by oligonucleotide-directed mutagenesis to encode sigma E directly. The sequence specifying the first amino acid of sigma E (GGC) was placed immediately downstream of the initiating codon (ATG) of P31. The resulting sigE allele (sigE...
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