Article
Identification of an amino acid on VP2 that affects neutralization of bluetongue virus serotype 10.
Virus research - 1 Aug 1994
Jewell J E, Mecham J O
Abstract excerpt
Genome segment 2, coding for the VP2 protein, of a neutralization resistant variant was compared to segment 2 of the bluetongue virus (BTV) serotype 10 parent from which the variant was derived. Full-length double-stranded cDNA of BTV segment 2 RNA, which was prepared by reverse transcription, was used as template to prepare overlapping subgenomic cDNA products by PCR. Purified PCR cDNA fragments were sequenced...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Base Sequence
- Bluetongue virus
- Capsid
- Capsid Proteins
- Codon
- DNA, Complementary
- Genetic Variation
- Molecular Sequence Data
- Neutralization Tests