Article
The N-terminal domain of VirG of Agrobacterium tumefaciens: modelling and analysis of mutant phenotypes.
Protein engineering - 1 Jul 1994
Rodenburg K W, Scheeren-Groot E, Vriend G, Hooykaas P J
Abstract excerpt
Fourteen mutants in the N-terminal domain of virulence factor G (VirG) were obtained by random mutagenesis. Two mutants showed an altered phenotype, all others were non-functional. All mutants can still be phosphorylated and bind to DNA. A 3-D model was built based on the coordinates of chemotaxi...
Topics
- Agrobacterium tumefaciens
- Amino Acid Sequence
- Bacterial Proteins
- Computer Simulation
- DNA-Binding Proteins
- Membrane Proteins
- Methyl-Accepting Chemotaxis Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Recombinant Fusion Proteins
- Sequence Alignment
- Structure-Activity Relationship
- Transcription Factors