Article
The N-terminal domain of VirG of Agrobacterium tumefaciens: modelling and analysis of mutant phenotypes.
Protein engineering - 1 Jul 1994
Rodenburg K W, Scheeren-Groot E, Vriend G, Hooykaas P J
Abstract excerpt
Fourteen mutants in the N-terminal domain of virulence factor G (VirG) were obtained by random mutagenesis. Two mutants showed an altered phenotype, all others were non-functional. All mutants can still be phosphorylated and bind to DNA. A 3-D model was built based on the coordinates of chemotaxis protein Y (CheY). Many of the observed phenotypic changes of VirG are explained qualitatively. Combination of model...
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