Article
PCR-amplification of simple sequence repeat variants from pooled DNA samples for rapidly mapping new mutations of the mouse.
Genomics - 1 Jun 1994
Taylor B A, Navin A, Phillips S J
Abstract excerpt
A novel strategy for rapidly mapping new mutations of the mouse is presented and tested. The inbred Mus musculus castaneus strain CAST/Ei is used as a tester stock. Separate DNA pools are made from mutant and wildtype intercross (F2) progeny and analyzed using simple sequence repeat (SSR) variants detected using PCR amplification. Linkage is indicated if the mutant DNA pool is enriched for the mutant-strain SSR...
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