PCR-amplification of simple sequence repeat variants from pooled DNA samples for rapidly mapping new mutations of the mouse.

doi:10.1006/geno.1994.1323

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Article

PCR-amplification of simple sequence repeat variants from pooled DNA samples for rapidly mapping new mutations of the mouse.

Genomics - 1 Jun 1994

Taylor B A, Navin A, Phillips S J

Abstract excerpt

A novel strategy for rapidly mapping new mutations of the mouse is presented and tested. The inbred Mus musculus castaneus strain CAST/Ei is used as a tester stock. Separate DNA pools are made from mutant and wildtype intercross (F2) progeny and analyzed using simple sequence repeat (SSR) variant...

Topics

Animals
Chromosome Mapping
Crosses, Genetic
DNA
Female
Genes, Recessive
Genetic Markers
Genetic Variation
Male
Mice
Mice, Mutant Strains
Models, Genetic
Mutation
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid

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